Characterization of recombinant endolysin from Vibrio parahaemolytics-infecting bacteriophage qdvp001

The inducible expression conditions of the recombinant endolysin Lysqdvp001 were discussed,founding that lowering temperature properly could avoid forming inclusion bodies. It was concluded that the recombinant endolysin Lysqdvp001 was induced by the addition of 1 mmol / L IPTG at 25 ℃ for 4 h. Under this condition,the Lysqdvp001 was soluble and was expressed in a high level.Then the Lysqdvp001 was characterized its lytic activity and biochemical properties such as p H,temperature and iron concentration by turbidity assay.The results showed that the Lysqdvp001 was stable over broad p H,maintaining good activities in p H3 ~ 10,and temperature ranges,maintaining 70% activity after 30 min at 75 ℃.Low iron concentration( 0.1 mmol / L) of Zn²⁺and Fe²⁺and different iron concentration( 10,1,0.1 mmol / L) of Ca²⁺could increase lytic activity of Lysqdvp001. In conclusion,the Lysqdvp001 had the potential to be antibacterial agents.