Application of response surface methodology on high yield methanobactin by Methylosinus trichosporium IMV 3011

To optimize Methanobactin( Mb) fermentation conditions for Methylosinus trichosporium 3011,Response Surface Methodology( RSM) were applied. The methanol flow,copper ion content and p H of seven factors were determined by Plackett- Burman design. The optimum condition was as follows: the methanol flow rate of54.1 μmol / min,the p H of 6.39,the copper content of 17.03 μmol / L,the incubation temperature of 30 ℃,the inoculation amount of 5 × 10~6 CFU / m L,the dissolved oxygen of 30 L / h,the stirring speed of 400 r / min.The optimum Mb production was 3300.2 μg / L. The RSM was feasible for high Mb production and this would improve the Mb production.