Antioxidant activities of polyphenols from pinus tabulaeformis pollen

The pinus tabulaeformis pollen polyphenols were extracted by ultrasonic with ethanol,and they were separated and purified by AB- 8 macroporous adsorption resin,the positive comparison was V_C ,the reducing power of pinus tabulaeformis pollen polyphenols and their scavenging effect on DPPH·,·OH,O₂^-·were researched by Fe³⁺- reducing method,DPPH·reducing method,salicylic acid colorimetry and pyrogallol autoxidation method.The experimental results showed that when the concentrations both were 6 μg / m L,pinus tabulaeformis pollen polyphenols was 16.33% significant higher than that of V_C on DPPH·clearance( p < 0.05),when the concentrations both were 8 μg / m L,pinus tabulaeformis pollen polyphenols was 32.03% significant higher than that of V_C on ·OH clearance( p < 0.05),when the concentrations both were 6 μg / m L,pinus tabulaeformis pollen polyphenols was10.53% unsignificant higher than that of V_C on O₂^-· clearance.When the concentrations both were 10 μg /m L,the absorbance of pinus tabulaeformis pollen polyphenols was 0.712 at 700 nm,was significantly higher than that of V_C ( A₍700 nm)= 0.353667,p < 0.05),so the reducing power of pinus tabulaeformis pollen polyphenols was higher than that of V_C . The results showed that pinus tabulaeformis pollen polyphenols had stronger reducing power and scavenging effectt on oxygen free radicals,and its clearance rate was higher than that of V_C . In conclusion,the pinus pollen polyphenols have significant antioxidant activity.