Effect of glucose concentration and feeding methods on hyaluronic acid fermentation

In this study,Streptococcus zooepidemicus( Streptococcus zooepidemicus) as the starting strain,the effect of glucose concentration and feeding methods on Hyaluronic acid fermentation was studied in 10 L automatic fermentation tank.The results showed that the total concentration of glucose should be controlled at 80 g / L,and both of the initial and supplemented glucose concentration was 40 g / L. The cell amount and production of hyaluronic acid were increased by 13.5% and 28.5% compared with the batch culture while the accumulation of lactic acid was reduced by 7.89%,the relative yield of hyaluronic acid was 10.02%,and average molecular weight was 1.56 × 10~6 u.Thus,the production of hyaluronic acid fermentation can be used single carbohydrate supplement cultured ways to enhance the intensity of production and product quality.