Study on separation of antiyeast active substances produced by Lactobacillus

The characteristics of antiyeast substances produced by Lactobacillus,which were isolated from traditional dairy fermentation food in Inner Mongolia were studied. The results were showed as follows: The active substances of ALAC- 3 and ALAC- 4 were separated and purified by G- 100 Sephadex gel filtration chromatography.And then the G100-3 and G100-5 were obtained respectively.They were analytically pure and the molecular weights were about 80.49 ku and 40.32 ku respectively through SDS- PAGE. G100- 3 and G100- 5 were judged separately as the single substances according to the thin- layer chromatography.