Effect of different drying methods on nucleoside,amino acids and flavonoids in the leaves of Ziziphus jujuba var.spinosa

To explore the effects of different drying methods on the quality of Ziziphus jujuba var.spinosa leaf( ZSL),the methods for determination of 20 nucleoside and 23 amino acids as well as rutin in ZSL were established by UPLC- TQ MS and HPLC method.Based on the results,the effects of different drying methods on the above constituents in the samples were evaluated.The results showed that the analysis method established in this study was simple,accurate and reliable.All samples were rich in nucleosides,free amino acids and rutin.The contents of phosphorylating products,such as cytidine 5'- monophosphate,adenosine 5'- monophosphate monophosphate and guanosine 5'- monophosphate were relative high in the nucleosides.For the free amino acids,high contents were found for proline,tyrosine and γ- aminobutyric acid in the samples.Also,the contents of these compounds determined in the samples after drying process were generally higher than those of fresh sample.Based on the contents of the compounds analyzed in the samples,TOPSIS analysis method was performed for evaluating the intrinsic quality of the samples dried by different methods.The results showed that the samples dried by the sun or hot air at 40 ℃ displayed better quality,while poor qualities were found for the fresh samples and the product dried at 70 ℃ with hot air.The above results provided the data for selecting the suitable drying process method of ZSL,and also could provide a reference for the drying process of leafy herbs.