Extraction optimization of crude polysaccharides from the seed cakes of Rhus succedanea L and its antioxidant activity

Single factor and orthogonal methodology were used to optimize the extraction conditions of crude polysaccharides from the seed cakes of Rhus succedanea L,then the antioxidant(scavenging hydroxyl and ABTS radicals) of the crude polysaccharides from the seed cakes of Rhus succedanea L were possessed. The optimum extraction conditions were as follows :extraction time 2.5 h,ratio of material to water 1 ∶20(g∶m L),extraction temperature 80 ℃,and extraction times 2. Under the optimized conditions,an experimental yield was 1. 561 %. The IC_(50) values scavenging hydroxyl and ABTS~+radicals of the crude polysaccharides were8.515 mg·m L~(-1) and 7.03 mg·m L~(-1),respectively. The hydroxyl radical scavenging ability of polysaccharides reached 61.5% at 25 mg·m L~(-1). The ABTS+radicals scavenging ability of polysaccharides reached 58.4% at10 mg·m L~(-1). The seed cakes polysaccharides displayed significant antioxidant.