Role of focal adhesion kinase in black rice anthocyanins inhibiting HER-2 positive human breast cancer cells

Objective: To explore the role of focal adhesion kinase in HER-2 positive human breast cancer cells antimetastasis by black rice anthocyanins( BRACs). Methods: To observe the effect of BRACs on the proliferation of MCF-10 A,MCF-2 and MDA- MB-453 cells with CCK8 method. Metastasis of MCF-10 A,MCF-2 and MDA- MB-453 cells were detected by wound healing experiments.The level of total and phosphorylated FAK and c Src were detected by western blot.The interaction between HER-2 and FAK were analyzed by immunoprecipitation Results:Different concentration of BRACs including 50,100,200,400 μg / m L were used to detect the influence which BRACs play on the growth MDA- MB- 453 cells. Compared with control,the inhibition ratio of each group were 18.74%,21.06%,22.82%,40.12% respectively. The migration distance of MDA- MB- 453 cells was decreased 37% by200 μg / m L BRACs. The level of phosphorylated FAK and c Src were reduced by BRACs and the interaction between HER- 2 / FAK was also decreased by BRACs. Conclusion: BRACs suppress the metastasis of HER- 2positive breast cancer cells via inhibiting the phosphorylation of FAK and c Src by decrease the interaction between FAK and HER-2 which maybe a mechanism of anti- metastasis by BRACs.