Optimization of extraction conditions and analysis of physicochemical property of pectin from Akebia trifoliata peel

Using Akebia trifoliata peel as the material,optimization of the acid extracting and alcohol precipitation process to extract pectin from Akebia trifoliate peel,and the degree of esterification and galacturonic acid of its physicochemical property were investigated. First of all,the effects of solid to liquid ratio,p H,extracting temperature and extracting time on the pectin yield were discussed by the single factor tests. And then,the orthogonal experiment was used to optimize the extraction conditions,and the degree of esterification and galacturonic acid were analyzed at last. The results showed that the optimum conditions of acid extraction and alcohol precipitation process of extracting pectin from Akebia trifoliate peel were as follows: solid to liquid- ratio1 ∶ 20( g / m L),p H1.5,extracting temperature 90 ℃,extracting time 120 min,and the extraction yield was 12.15 %under these conditions.The pectin from Akebia trifoliata peel was a kind of high methoxyl pectin,its degree of esterification was 81.94%,and its galacturonic acid was 83.17%,meet GB 25533- 2010 of galacturonic acid content requirements.