Development of a direct competitive chemiluminescent enzyme immunoassay for detection of furaltadone metabolite

A direct competitive chemiluminescent enzyme immunoassay method( dc-CLEIA) for detection of AMOZ which based on lodine phenol enhancer Luminol-HRP-H2O2 system was developed. The 3-amino-5-morpolinomethyl-2-oxazolidinone was metabolite of furaltdone. The sensitivity(IC50) and detection limit(LOD)of the method were 0.62 ng/m L and 15.52 pg/m L respectively. The linear range was 0.038 ~9.78 ng/m L. The relative standard deviations was below 10%. The antibody was high specific for furaltdone derivative and no cross-reactivity except the furaltdone original drug. The accuracy of the method was verified and the recovery in chicken tissues was 83%~94%. The method provided a convenient,accurate and rapid screening means for detecting AMOZ in actual animal tissue.