Study on the preparation of antioxidant peptides from Schizochytrium limacinum by composite enzymatic hydrolysis

In this paper,Schizochytrium limacinum after oil extraction was hydrolyzed while the degree of hydrolysis,protein recovery and product antioxidant activity were as index to determine the best complex enzymatic processes. The results showed that the optimum conditions were as follows:p H7.5,temperature 50 ℃,ratio of material to water 1∶12,time 4 h,Protamex and Alcalase 2.4 L complex method,total amount of enzyme dosage0.5%(E/S,w/w,1∶1),which was significantly better than the effect of single enzymatic hydrolysis. In this condition,the hydrolysates had the degree of hydrolysis of 11.15%,protein recovery 70.82%. In addition,IC50 of hydroxyl radicals was 1.656 mg/ m L and reducing power was 1.417.