Optimization of fermentation medium for lutein degradation applying response surface methodology

Optimization of fermentation medium using for lutein degradation to form important aroma compounds with Pantoea dispersa Y08 strain was performed employing the response surface analysis ( RSA) method.Based on single factor experiment, three critical factors ( the content of sucrose, mixed nitrogen sources and KH2PO4) were selected as response factors.The quadric regression equation was established according to the lutein degradation yield.As a result, the optimum fermentation medium was composed of sucrose 0.97%, mixed nitrogen sources ( yeast extract∶asparagine = 2∶1) 1.38% and KH2PO40.15%.Under the situation, the highest yield of degradation of lutein was predicted to be 80.03%. After optimization, the lutein degradation yield was increased by 140.67%. The experimental values were in according with the predicted values.