Study on optimization on composite enzyme extraction process of inulin from Cichorium intybus L. by response surface design

In this experiment,with chicory root as a raw material,pectinase and cellulase compound preparations was used to extract inulin. Four extraction parameters including the ratio of material and solvent,extraction temperature,extraction time and extraction p H were optimized as the result of the conditions for maximum extraction of inulin from Cichorium intybus L. by Box-Behnken response surface design methodology based on the inulin extraction rate as a response value. Results showed that the optimum condition of inulin extraction was as follows the ratio of material and solvent 1 ∶8g/m L,extraction temperature 60℃,extraction time 2.5h,extraction p H6.0,and the ratio of compound enzymes 1∶2. Under this condition inulin extraction rate was up to54.63%. The extraction rate increased by 64.10%,compared with the method of hot water extraction.