Study on comparison of three extraction methods of traditional fermented yak yogurt macro genomic DNA

Microflora was so complex that around which 95% could not be obtained by culture in conventional way. This paper selected the traditional fermented yak yogurt,and extracted the macro genomic DNA by three methods respectively,including CTAB-SDS-frozen-thawed,liquid nitrogen trituration and glass bead adsorptive process. Then the DNA concentration,purity and fragment distribution,and the total DNA of 16 S r RNA gene PCR amplification was tested.The result showed that all the three methods could extract the DNA suitable for the follow-up study. But liquid nitrogen trituration method not only had the priority of a better DNA quality,but also was simplest and lowest costed way compared to the other two methods. So it was the most suitable method to extract yak yogurt macro genomic DNA.