Separation and purification of triterpenes from Matteuccia struthiopteris with macroporous resin

Objective:An optimal macroporous resin and process that to separate and purify triterpenes from Matteuccia struthiopteris was reasearched. Methods : The optimal resin and purificating technology were preliminary selected through the static and dynamic test, taking the concentration of triterpenes as the main index. Results:The best macroporous resin was AB-8, the adsorption conditions were described as follows:the p H and concentration of sample was 6 and 1. 96 mg / m L, the maximum injection volume was 5BV, the flow velocity was 1.5m L/min. The desorption process was determined as follows:60% ethanol was used as eluent at the flow rate of 2.0m L/min with 4BV, the recovery rate reached up to 86.27%, the purity was 82.32%, the purified multiples of triterpenes was 2.88. Conlusion:These data demonstrated the AB-8 macroporous resin in purifying triterpenes from Matteuccia struthiopteris was well. This method was easy to operate and displayed a good purification effect, the certain reference and theoretical guidance for separation and purification of triterpenes in industrial application could be provided.