Analysis of catechin, caffeine and gallic acid in Kangzhuan tea by HPLC-DAD

A High- performance Liquid Chromatographyhigh method with a diode array detector ( DAD) and AcclaimTM120 as column, was established for simultaneous analysis of tea catechins, caffeine (CAF) , and gallic acid (GA) . Its chromatographic conditions were as follows :mobile phase of KH2PO4 and acetonitrile, gradient elution, flow rate of 1.0m L/min, temperature of column 25℃, sample amount 10μL, Detector wavelength 278 nm.The results showed that six kinds of catechins, caffeine, gallic acid were in a range of good linearity correlation coefficient of 0.9991 to 0.9998, with low intra-and inter deviation, the average recovery between 92.49% ¹06.08%, and the relative standard deviation (RSD) of less than 3.59%. Futhermore, This method was applied to analyze Kangzhuan tea, its GA, EGC, CAF, C, EC, EGCG, EGCG3"Me, ECG contents were between 0.0717% ¹.115%, 0.280%⁰.660%, 2.728%⁴.621%, 0⁰.0882%, 0.157%¹.238%, 0.343%⁷.176%, 0⁰.172% and0.108%¹.654%, respectively.