Study on the antioxidant activity and protective effect on mitochondria of Lycium ruthenicum functional ingredients

XIA Na; ZHAO Li-feng

doi:10.13386/j.issn1002-0306.2014.22.027

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Science and Technology of Food Industry > 2014 > (22): 162-166. > DOI: 10.13386/j.issn1002-0306.2014.22.027

XIA Na, ZHAO Li-feng. Study on the antioxidant activity and protective effect on mitochondria of Lycium ruthenicum functional ingredients[J]. Science and Technology of Food Industry, 2014, (22): 162-166. DOI: 10.13386/j.issn1002-0306.2014.22.027

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Study on the antioxidant activity and protective effect on mitochondria of Lycium ruthenicum functional ingredients

The Key Laboratory of Ecology and Biological Resources in Yarkand Oasis at Colleges & Universities under the Department of Education of Xinjiang Uygur Autonomous Region

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Received Date: March 13, 2014

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Objective : To study the antioxidant activity of Lycium ruthenicum ( polysaccharide, flavonoids and polyphenols) extracts, as well as protective effect on mitochondrial. Methods:Polysaccharides, flavonoids and polyphenols were extracted from Lycium ruthenicum Murr. Comparison of their antioxidant activity was carried out by scavenging of DPPH radical, hydroxyl free radical (·OH) and superoxide anion radical (O2-·) . The inhibit effects of Lycium ruthenicum extracts on liver mitochondria swelling and malondialdehyde (MDA) content were measured. Results :Lycium ruthenicum contains abundant polysaccharide, flavonoids and polyphenols, the contents of polysaccharide, flavonoids and polyphenols were 30.6mg/g, 21.3mg/g and 49.5mg/g of dry Lycium ruthenicum, respectively. The free radical scavenging capacity of flavonoids and polyphenols from Lycium ruthenicum were stronger than that of polysaccharides. The free radical scavenging capacity of polysaccharides, flavonoids and polyphenols were 52.5%, 72.4% and 82.3% against DPPH free radicals, ·OH 48.2%, 56.6% and75.8% against ·OH, 16.1%, 40.7% and 53.3% against O2-·, respectively. Flavonoids (p<0.01) and polyphenols (p<0.01) from Lycium ruthenicum were significantly inhibit mitochondria swelling caused by Fe SO4 and VC, and flavonoids and polyphenols can significantly reduce the mitochondrial MDA content ( p <0.05) . Conclusion :Extracts from Lycium ruthenicum (polysaccharides, flavonoids and polyphenols) had antioxidant activity and mitochondrial protection, flavonoids and polyphenols protection stronger than the polysaccharides of mitochondria.
- Lycium ruthenicum,
- polysaccharide extract,
- flavonoid extract,
- polyphenols extract,
- mitochondria

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[1]	甘青梅.浅述藏药的研究[J].中草药, 2001, 32 (4) :371-373.
[2]	刘永民.维吾尔药志 (下) [M].乌鲁木齐:新疆科技卫生出版社, 1999:478-485.
[3]	李进, 李淑珍, 冯文娟, 等.黑果枸杞叶总黄酮的体外抗氧化活性研究[J].食品科学, 2010 (13) :259-262.
[4]	李进, 瞿伟菁, 张素军, 等.黑果枸杞色素的抗氧化活性研究[J].中国中药杂志, 2006, 31 (14) :1179-1183.
[5]	汪河滨, 白红进, 王金磊, 等.黑果枸杞色素清除自由基活性的研究[J].食品研究与开发, 2006, 27 (11) :8-10.
[6]	李彩霞, 焦扬, 梁倩倩, 等.黑果枸杞提取物抗氧化性研究[J].食品工业科技, 2006, 27 (10) :55-57.
[7]	汪建红, 陈晓琴, 张蔚佼.黑果枸杞果实多糖降血糖生物功效及其机制研究[J].食品科学, 2009, 30 (5) :244-248.
[8]	李淑珍, 李进.黑果枸杞总黄酮降血脂作用[J].时珍国医国药, 2012, 23 (5) :1072-1074.
[9]	Aruoma O I.Free radicals, oxidative stress, and antioxidants in human health and disease[J].Journal of the American Oil Chemists’Society, 1998, 75 (2) :199-212.
[10]	Fridovich I.Superoxide anion radical (O2·-) , superoxide dismutases, and related matters[J].Journal of Biological Chemistry, 1997, 272 (30) :18515-18517.
[11]	Floyd R A.DNA-ferrous iron catalyzed hydroxyl free radical formation from hydrogen peroxide[J].Biochemical and Biophysical Research Communications, 1981, 99 (4) :1209-1215.
[12]	Inoue M, SATO E F, NISHIKAWA M, et al.Mitochondrial generation of reactive oxygen species and its role in aerobic life[J].Current Medicinal Chemistry, 2003, 10 (23) :2495-2505.
[13]	Wu J L, Wu Q P, Peng Y P, et al.Effects of L-malate on mitochondrial oxidoreductases in liver of aged rats[J].Physiol Res, 2011, 60:329-336.
[14]	Sheu S S, Nauduri D, Anders M W.Targeting antioxidants to mitochondria:a new therapeutic direction[J].Biochimica et Biophysica Acta (BBA) -Molecular Basis of Disease, 2006, 1762 (2) :256-265.
[15]	涂宗财, 李敏, 刘光宪, 等.苯酚-硫酸法测定油菜花粉多糖含量的研究[J].食品工业科技, 2007, 28 (4) :219-221.
[16]	盛清, 任玉翠.鱼腥草制剂中总黄酮含量的测定[J].食品工业科技, 1999, 20 (1) :64-65.
[17]	Singleton V L, Rossi J A.Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents[J].Am J Enol Vitic, 1965, 16 (3) :144-158.
[18]	Amarowicz R, Naczk M, Shahidi F.Antioxidant activity of various fractions of non-tanin phenolics of canola hulls[J].J Agric Food Chem, 2000, 48 (7) :2755-2759.
[19]	文良娟, 毛慧君, 张元春, 等.西番莲果皮成分分析及其抗氧化活性的研究[J].食品科学, 2008, 29 (11) :54-58.
[20]	Liang X L, Wang X L, Li Z, et al.Improved in vitro assays of superoxide anion and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity of isoflavones and isoflavone metabolites[J].Journal of Agricultural and Food Chemistry, 2010, 58 (22) :11548-11552.
[21]	韩玲, 陈可翼.益心康胶囊含药血清对氧应激所致线粒体损伤的保护作用[J].中国实验方剂学杂志, 2001, 7 (3) :25-28.
[22]	陈晨, 赵晓辉, 文怀秀, 等.黑果枸杞的抗氧化成分分析及抗氧化能力测定[J].中国医院药学杂志, 2011, 31 (15) :1305-1306.
[23]	Attardi G, Schatz G.Biogenesis of mitochondria[J].Annual Review of Cell Biology, 1988, 4 (1) :289-331.
[24]	Green D R, Reed J C.Mitochondria and apoptosis[J].ScienceAAAS-Weekly Paper Edition, 1998, 281 (5381) :1309-1311.
[25]	Zorov D B, Juhaszova M, Sollott S J.Mitochondrial ROSinduced ROS release:an update and review[J].Biochimica e t Biophysica Acta (BBA) -Bioenergetics, 2006, 1757 (5) :509-517.
[26]	Rasbach K A, Schnellmann R G.Signaling of mitochondrial biogenesis following oxidant injury[J].Journal of Biological Chemistry, 2007, 282 (4) :2355-2362.

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