Purification of recombinant antihypertensive peptides VLPVPR by gel and ion exchange chromatography

To purify the peptide VLPVPR, gel filtration combined with ion exchange chromatography method was employed.Sephadex G-10 was used to desalt firstly.Compared the purification effective of SP Sepharose Fast Flow and Q Sepharose Fast Flow.SP Sepharose Fast Flow was not suitable for VLPVPR purification while Q Sepharose Fast Flow was suitable for VLPVPR purification.The optimization condition of Q Sepharose Fast Flow were pH9.0, feeding volume 10% BV, CHES concertration 10 mmol /L, elute rate 1.0mL /min.The recovery ratio was 93.8% and the purity ratio was 47.2%.The purification effective of Q Sepharose Fast Flow was improved.