Quantitative analysis of ganoderic acid T, S and R in Ganoderma mycelium by HPLC

An HPLC-based method for the accurate quantitative analysis of GA-T, GA-S and GA-R in different Ganoderma mycelium was described. The HPLC method was performed on YMC C18column (250mm×4.6mm, 5μm) , with 0.5% acetic acid (A) -methanol (B) as mobile phase (0~20min, 85%B→100% B, 20~30min, 100% B) .UV detection wavelength was 245 nm. The flow rate was 1mL/min. The column temperature was 30℃. The result suggested significant differences in analyte ganoderic acid content among the different G. lucidium strains, and the significance level was 0.0003. The method was proved to be convenient, stabilized and repeatable, so it could be used for the quantitative analysis of three ganoderic acids in Ganoderma mycelium.