Optimization of microwave extraction technologies of flavonoids from Coriandrum sativum L.with Plackett-Burman design and response surface analysis

In order to obtain the optimum extraction conditions of total flavonoids from Coriandrum sativum L., the factors affecting the extraction rate of total flavonoids were evaluated by Plackett-Burman Design firstly.Three main factors including the ratio of solvent to sample, the microwave extraction temperature, and the ethanol concentration were selected. These factors were optimized by Response Surface Methodology ( RSM) , the extraction rate of total flavonoids was chosen as the response. The results showed that the optimal extraction conditions were as follows: the ratio of solvent to sample was 35∶1, the microwave extraction temperature was 80℃, and the ethanol concentration was 67%.Under the optimal extraction conditions, the predicted value of the rate of total flavonoids was 1.33%, the measured value was 1.31%, and the deviation was 1.30%.The result indicated that the extraction technology of total flavonoids from C.sativum could be optimized well by the combination of Plackett-Burman Design and RSM.