Study on optimization of Ca2+on activation of prothrombin process conditions in yak blood by response surface method

Yak blood as the research object, the prothrombin was prepared by adsorption method with barium citrate. The study optimized preparation conditions of Ca2 +activating the prothrombin in yak blood by single factor experiment (the activating temperature, final concentration of Ca2+and activating time) and Box-Behnken experiment. The results showed that the optimal thrombin specific activity was 63.3972U/mg at the following conditions:activating temperature 27℃, final concentration of Ca2+0.01mol/L, activating time 1.95h.