Extraction and identification of phenolic constituents in Schisandra chinensis ( Turcz.) Bail berry

The phenolic constituents yield of S. chinensis berry was chosen as indicators. The optimal extraction conditions of leaching extraction were studied and the major phenolic ingredient in the extract was identified.Single factor and uniform test were used to determine the optimal extraction process of phenolic constituents.The extract was purified and analyzed by UV spectrophotometry and pH color response. HPLC /MS/MS technique was employed to identify the major phenolic constituents.The results showed that the optimal extraction conditions of phenolic constituents were extraction time 45min, liquid- solid ratio 6 ∶1, optimal yield 1.085%. The major phenolic component was identified as cyanidin-3-O-xylosylrutinoside, which was also the main red pigment of S.chinensis berries.