Study on separation and purification of ACE inhibitory peptides from Apios americana Medikus

In the present paper, Apios americana Medikus root was used as the raw material to produce polypeptide by enzyme hydrolysis method and its ACE inhibitory activity was investigated. To purify the ACE inhibitory component from Apios americana Mediku peptides, the enzyme hydrolysis products were separated in turn by ultrafiltration, macroporous resin desalination, anion exchange chromatography and gel chromatography. Results showed that the component IV which had 62.32% ACE inhibitory rate was obtained after the ultrafiltration process of the enzymolysis liquid.After desalinating with DA201- C macroporous resin, a component with a ACE inhibitory rate of 66.65% was purified from the component IV.The component was further purified with anion exchange resin, DEAE-Sepharose Fast Flow.The component G3 showed highest ACE inhibitory activity with IC50value 66.00μg /mL.The component G3 was further separated by using Sephadex G-15 gel chromatography and the component F2was obtained, which showed a high ACE inhibitory activity with a IC50value of 37.47μg /mL.