Preparation of high purity capsaicin from Capsicum chinense

Capsicum oleoresin was used as the raw material to explore the effect of resin separation and crystallization on the purity of capsaicinoids. The results showed that NKA-Ⅱ macroporous resin was feasible for the purification. During the resin adsorption process, the optimum adsorption conditions were as follows:4.0mg/mL of the sample concentration, 2.5mg/mL of the sample flow rate with pH7. And the optimal desorption conditions were 90% of alcohol, 2.0mL/min of the eluting rate with pH4. After capsicum oleoresin was separated by NKA-Ⅱresin and extracted by petroleum ether and ethyl ether, then it was recrystallized by petroleum ether for 3.0h with 20mg/mL of concentration and-5℃ of temperature, the purity of capsaicin was up to 92.46%.