Separation of the greening compounds in garlic by macroporous resin SIPI-40

Garlic greening was a common phenomenon in industry and day life. Study on the greening compounds by purification has great importance. Resin SIPI-40 was chosen as the most effective separation media, and the optimal separation condition of column chromatography was :injecting volume 2BV, injecting velocity 1.0mL/min, 90% ethanol with 0.1% HCl as eluent, eluting volume 4BV. The color value of the purified pigment extract was elevated to 47.6. Seperation by macroporous resin SIPI-40 was a cheap, quick and effective pretreatment method for further accurate purification.