Study of the method validation of testing saccharin sodium salt in mooncake filling

The purpose was to validate the method of tesing saccharin sodium salt in mooncake filling by HPLCMS/MS.The mobile phase, flow rate, sample size and many parameters of MS- MS were optimized.Some random mooncake filling were detected.Comparing with HPLC, the superiority of this method was avoiding false positive and miscarriage of justice.The developed method exhibited good linearity over the range from 0.01mg /L to 10.00mg /L with a correlation coefficient of 0.9992.The limit of quantification was 0.2mg /kg.The method was simple with high sensitivity, selectivity and precision, which was suitable for quick determination of trace saccharin sodium salt in mooncake filling.