Separation and identification of Leuconostoc during the fermentation of sichuan pickles

The Leuconostoc throughout spontaneous fermentation of three kinds of Sichuan pickles were analyzed. Four strains of different colony morphology were separated, and named MC-1, MC-2, MC-13, J-5. J-5 only existed in the bean pickle, others existed in all kinds of pickles. After some physiological and biochemical tests and sequencing the 16SrDNA sequence of the four strains, MC-1 was identified as L.lactis, and MC-2 was L.mesenteroides subsp.dextranicum, MC-13 was L.citreum and J-5 was L.pseudomesenteroide. Dynamic results showed that L.lactis and L.mesenteroides subsp.dextranicum were the dominant Leuconostoc, they presented in the brine as soon as vegetables pickled, L. citreum presented in the 2 ~ 4th day and disappeared soon, L.pseudomesenteroide existed in 3⁹th day. All the L.lactis disappeared till the 11th day.