Optimization of ultrasound-assisted extraction of polysaccharides from Avicennia marina by response surface analysis

In order to optimize ultrasonic extraction of polysaccharides in Avicennia marina, a mathematical regression model ( water /material ratio, extraction temperature ultrasonic time and extraction times as the factors, the yield of polysaccharides as index) for predicting extraction yield of polysaccharides was established by fitting Box-Benhnken central composite design experimental results.The optimal protocol for polysaccharides extraction from Avicennia marina was based on one time extraction for 46min at 100℃ with a ratio of water to dry material of 49∶1, the optimum extracting rate for the Avicennia marina was 4.76%.