Optimization of conditions of nitrite reductase production in Pediococcus pentosaceus from salted fish

Conditions for nitrite reductase produced in Pediococcus pentosaceus from salted fish were optimized.Based on single factor tests, four factors including inoculate quantity, pH, culture temperature and incubation time were selected as influencing factors by the use of L9 (34) orthogonal experiment design, enzyme activity of nitrite reductase was used as evaluation index.Results showed that the inoculate quantity had greatest influence on enzyme activity.The optimum condition of the Pediococcus pentosaceus nitrite reductase produced were in inoculate quantity of 4%, pH value of 6, culture temperature of 35℃, the incubation time for 48h.It was available for further study of nitrite reductase properties and application in the salted fish processing, in order to reduce the dry salted fish and pickled fish products by nitrite and nitroso compounds to provide technical support.