Construction of genetically engineering Escherichia coli for resveratrol production

To construction of expression plasmid pMD18- Vvrs- At4cl by touchdown overlap extension PCR and ligase connection.The constitutive vector containing a gap promoter (pGAP) of Escherichia coli and 4- coumaroyl:CoA ligase gene of Arabidopsis thalianna (At4CL) and resveratrol synthase gene of Vitis vinifera, (VvRS) , which transformed into host strain E.coli BW27784.Next, with fermentation and HPLC detection, created an recombination E.coli strain caple of bioproducing resveratrol by additionally supplemented with p-coumaric acid and resulted in a final titer of 4.51mg /L resveratrol.