Optimization of xiangyuan (C.wilsonii Tanaka) pectin extraction via response surface method

For the optimization of pectin extraction from xiangyuan (C.wilsonii Tanaka) peel using hydrochloric acid extraction and ethanol precipitation method, a Box- Behnken design (BBD) was applied based on single factor experiments.Four independent variables (liquid- solid ratio, pH, extraction temperature and time) on the yield of pectin were evaluated.Results showed that pH was the most important factor followed by extraction temperature, then liquid-solid ratio and extraction time.The maximum yield of pectin (15.52%) was found when the liquid- solid ratio was 25mL /g, and hydrolyzed at pH2.0, 85.0℃ for 1.75h.Under the optimal conditions, the experimental values agreed with the predicted values by analysis of variance.