Promoter P43 regulatory effect for subtilosin A synthesis by expressing key enzyme gene

For improving Bacillus subtilis 168 bacteriocin subtilosin A production and applying to food preservation, medical science and so on, P 43 was linked to key enzyme gene sbo-albA, albB-albC of subtilosin A operon using SOE-PCR.High expressing vector was constructed.This recombinant vector was transformed into Bacillus subtilis 168 by high osmolarity electroporation method.HPLC assay showed subtilosin A production was improved.Inhibited method also showed the improving.This method will provide theory reference of improving subtilosin A production and applying in productive practice.