Optimization of activation condition of prothrombin from horse blood by using response surface methodology

Based on single factor experiment, three main factors were screened out, including activation time, activation temperature, and concentration of Ca2 +. Optimization of these factors by using response surface methodology, the results showed that the optimum activation conditions of prothrombin were as follows:activation time 2.8h, temperature 32℃, concentration of Ca+20.11mol/L. Under these conditions, the specific activity of thrombin was 51.16U/mg.