LU Ji-ke, GU Guo-feng, WANG Rui, HU Tian, HAO Li-min, YU Jian-yong, WANG Ying-ze. Optimization of extraction of polysaccharide from Cordyceps sobolifera[J]. Science and Technology of Food Industry, 2013, (22): 196-199. DOI: 10.13386/j.issn1002-0306.2013.22.040
Citation: LU Ji-ke, GU Guo-feng, WANG Rui, HU Tian, HAO Li-min, YU Jian-yong, WANG Ying-ze. Optimization of extraction of polysaccharide from Cordyceps sobolifera[J]. Science and Technology of Food Industry, 2013, (22): 196-199. DOI: 10.13386/j.issn1002-0306.2013.22.040

Optimization of extraction of polysaccharide from Cordyceps sobolifera

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  • Received Date: August 15, 2013
  • Extraction of polysaccharide from Cordyceps sobolifera in Zhejiang was investigated, and the calculation method of the extraction yield was determined. With water as extraction solvent, the effect of extraction temperature, extraction time, solid-liquid ratio and extraction times on the yield of polysaccharide were optimized with single factor test followed by four factor-four level orthogonal experiment. The result showed that the effect of the investigated factors on the polysaccharide extraction yield was in the order as extraction temperature>extraction times>solid-liquid ratio>extraction time. The optimal extraction conditions of polysaccharide were that extraction temperature of 90℃, solid-liquid ratio of 1∶40 (g∶mL) , extraction time of240min and extraction 4 times. Under these conditions the polysaccharide extraction yield was 11.82%.
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  • [1]
    许胜杰, 叶淑幸, 陈劲初.新资源食品蝉花菌丝体的开发[J].食药用菌, 2011, 19 (6) :34-38.
    [2]
    温鲁, 唐玉玲, 张平.蝉花与有关虫草活性成分检测比较[J].江苏中医药, 2006, 27 (1) :45-46.
    [3]
    官宗华, 宋玉良.蝉花的研究概况[J].中国中药指南, 2012, 10 (10) :443-444.
    [4]
    Weng S-C, Chou C-J, Lin L-C, et al.Immunomodulatory functions of extracts from the Chinese medicinal fungus Cordyceps cicadae[J].Journal of Ethnopharmacology, 2002, 83 (1-2) :79-85.
    [5]
    Wu M-F, Li P-C, Chen C-C, et al.Cordyceps sobolifera extract ameliorates lipopolysaccharide-induced renal dysfunction in the rat[J].The American Journal of Chinese Medicine, 2011, 39 (3) :523-535.
    [6]
    俞滢, 顾蕾, 陈启琪, 等.蝉花的化学成分分析[J].杭州师范学院学报, 1997 (6) :61-63.
    [7]
    余佳文.蝉花中多球壳菌素类长链碱的测定方法学研究及代谢轮廓分析[D].重庆:重庆大学, 2009.
    [8]
    Wang S-X, Liu Y, Zhang G-Q, et al.Cordysobin, a novel alkaline serine protease with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Cordyceps sobolifera[J].Journal of Bioscience and Bioengineering, 2012, 113 (1) :42-47.
    [9]
    徐晓飞, 陈健.多糖含量测定的研究进展[J].食品科学, 2009, 30 (21) :443-448.
    [10]
    何晓波, 芦柏震, 周俐斐, 等.蝉花总多糖对细胞免疫和体液免疫反应的促进作用[J].中华中医药学刊, 2010, 28 (7) :1465-1468.
    [11]
    朱燕, 程东庆.蝉花多糖抗氧化活性的测定[J].中华中医药学刊, 2009, 27 (7) :1552-1554.
    [12]
    姚婷, 罗靖, 宋捷民.金蝉花多糖的微波辅助提取工艺研究[J].江西中医学院学报, 2012, 24 (5) :73-76.
    [13]
    余文娟, 许明敏, 潘丽辉, 等.蝉花多糖超声波辅助提取工艺的优化[J].湖北农业科学, 2012, 51 (20) :4598-4600.
    [14]
    周俐斐, 芦柏震, 蔡菊芬.蝉花总多糖的提取纯化及含量测定[J].江西中医药, 2009, 40 (1) :56-57.
    [15]
    郝利民, 张建春, 鲁吉珂, 等.云南种植玛咖中多糖提取工艺的优化研究[J].食品工业科技, 2013 (3) :262-264.

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