Study on enzymatic characteristics of Polyphenol Oxidase ( PPO) in pomelo
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Graphical Abstract
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Abstract
Using catechol as substrates, the characteristics of polyphenol oxidase ( PPO) extracted from pomelo were studied by spectrophotometer. The results showed that the maximum absorption wavelength of PPO was422nm, the optimum range of temperature and pH of PPO were 5070℃ and 6.06.6 respectively.K m was 0.947mol /L, V max was 0.375U /min.The PPO was inactivated when the temperature was higher than 80℃.
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