Study on protein removal technology of asparagus polysaccharide

With retention rate and deproteinized rate of polysaccharide as comprehensive evaluation indexes, to determine the process conditions of two methods including Sevage, TDA- NBA by the uniform design experiment. Results showed that optimum conditions were as follows:Sevage and feed liquid volume rate was1 ∶2, chloroform and NBA volume rate was 3 ∶1, oscillating time was 34min, standing time was 36min, deproteinized rate retention rate and of polysaccharide were 85.92%±0.15%, 42.68%±0.10%, respectively. TDA and NBA volume rate was 1 ∶20, TDA-NBA and polysaccharide solution volume rate was 1 ∶2, oscillating time was 39min, standing time was 60min, deproteinized rate retention rate and of polysaccharide were 66.21% ±0.11%, 91.49%±0.30%, respectively. Results showed that TDA-NBA was better than Sevage.