Determination of L-citrulline in watermelon by high performance liquid chromatography with PITC precolumn derivation
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- Received Date:
March 26, 2013
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Abstract
A method of RP-HPLC with phenylisothiocyanate (PITC) precolumn derivatization was established for the determination of L-citrulline in watermelon. The calibration curves of L-citrulline were linear over the range of 4.26213.2μg/mL (R=0.9998) and the recovery rate was 98.1%. The content ranges of L-citrulline in watermelon were 1.132.71mg/g. The detection limit of the method was 1.1μg/mL. The method descried in this paper was fast, sensitive and reliable and it was most suitable for quantitative determination of L-citrulline in watermelon.
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References
| [1] |
戴思慧, 孙小武, 熊兴耀, 等.西瓜综合利用研究进展及深加工发展趋势[J].中国园艺文摘, 2010 (10) :57-59.
|
| [2] |
LIU Wen-ge, YAN Zhi-hong, ZHAO Sheng-jie, et al.Triploid seedless watermelon production in China[J].Cucurbitaceae, 2006 (5) :296-300.
|
| [3] |
万学闪, 刘文革, 阎志红, 等.无籽西瓜果实不同部位瓜氨酸含量测定[J].中国瓜菜, 2010, 23 (6) :11-14.
|
| [4] |
程志强, 刘文革, 邓云, 等.西瓜果实中L-瓜氨酸的提取与测定[J].果树学报, 2010, 27 (4) :650-654.
|
| [5] |
Cheng Bai, Charles C Reilly, Bruce W.Wood.Identification and Quantitation of Asparagine and Citrulline Using HighPerformance Liquid Chromatography (HPLC) [J].Anal Chem Insights, 2007 (2) :31-36.
|
| [6] |
Li Jiayou, CaoYu, Liu Qian.Determination of urinaryLcitrul-line by enzymatic method[J].Chinese Journal of Analytical Chemistry, 2006, 34 (3) :379-381.
|
| [7] |
李加友, 曹瑜, 焦庆才.酶法转化液中L-瓜氨酸的分光光度法测定[J].分析实验室, 2005, 24 (12) :8-11.
|
| [8] |
岳瑞, 歧琳, 傅强.双波长薄层扫描法测定天花粉中瓜氨酸的含量[J].西北药学杂志, 2002, 17 (3) :107-111.
|
| [9] |
周慧, 郝宁, 严明, 许琳.柱前衍生化HPLC法测定发酵液中L-瓜氨酸和L-鸟氨酸含量[J].南京工业大学学报, 2009, 31 (2) :77-80.
|
| [10] |
AGNES M R, PERKINS P M.Determination of citrulline in watermelon rind[J].Journal of Chromatography A, 2005, 1078:196-200.
|
| [11] |
牟昳, 袁其朋, 翁南梅, 等.高效液相色谱分析L-瓜氨酸的研究[J].北京化工大学学报, 2004, 31 (3) :110-112.
|
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