Study on optimization of extraction technique of the insoluble dietary fiber from dried osmund using response surface methodology

The dried osmund was used as the raw materials in this paper.The technology for extracting insoluble dietary fiber of α-amylase and alkaline hydrolysis were studied using response surface methodology.Results showed that the optimized conditions of α-amylase enzymolysis were α-amylase content of 0.7%, pH of 5.8, the enzymolysis temperature 35℃, the enzymolysis time 1.0h.The yield rate of enzymolysis was 60.68%.And the optimized conditions for the alkaline hydrolysis were showed as follows:sodium hydroxide concentration of 0.5mol/L, temperature of 65℃, time of 2.0h, material/liquid ratio (g/mL) of 1∶10.The yield rate of insoluble dietary fiber was 38.86%.