Study on extraction and detection of folic acid in millet

SHAO Li-hua; WANG Li; BAI Wen-wen; LIU Ya-juan

doi:10.13386/j.issn1002-0306.2013.18.070

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Science and Technology of Food Industry > 2013 > (18): 253-256. > DOI: 10.13386/j.issn1002-0306.2013.18.070

SHAO Li-hua, WANG Li, BAI Wen-wen, LIU Ya-juan. Study on extraction and detection of folic acid in millet[J]. Science and Technology of Food Industry, 2013, (18): 253-256. DOI: 10.13386/j.issn1002-0306.2013.18.070

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Study on extraction and detection of folic acid in millet

Life Science College of Shanxi Normal University

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Received Date: April 09, 2013

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Abstract

The optimum extraction condition and measurement method for analysis of folic acid in millet was studied.The red valley millet cultivar from Hebei was used as materials, and a single factor experiment for folic acid extraction and detection was respectively conducted based on the illumination condition, color rendering time, detection ranges, extraction time, usage of absorbent, the consumption amount of elution solution as well as the recovery rate of standard sample.Data showed that 25mL of 0.1mol/L KH2 PO4 was used to obtain the optimum extraction effect by soaking 2.5g millet at 50℃ for 8 hours, and adsorbing for 10 minutes with 0.5g active carbon adsorbent treated by aniline, and continuously eluting five times with 7mL of mixture containing 3% ammonia and 70% ethanol, and then indirect fluorescent method was used to determine the contents of folic acid oxidized by KMnO4, and the satisfactory result was obtained because of higher the recovery rate of 96.14% with a relative lower deviation of 1.68%.
- millet,
- folic acid,
- extraction,
- measurement,
- indirect fluorescent method

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[1]	韩月婷, 姜凌, 范云六, 等.叶酸生物强化研究进展[J].中国农业科技导报, 2009, 11 (4) :23-28.
[2]	孙维洋, 李剑芳, 牟志美.植物叶酸的合成、代谢及基因工程研究进展[J].安徽农业科学, 2008, 36 (6) :2229-2230.
[3]	王小娟, 王振林, 肖生彬, 等.陕西中农村地区两所中学学生贫血原因的探究[J].西安交通大学学报, 2008, 29 (5) :530-534.
[4]	张娟, 靳保辉, 刘灵芳, 等.差示脉冲溶出法测定粮食中叶酸的研究[J].郑州工程学院学报, 2001, 22 (2) :91-94.
[5]	李富兰, 颜杰, 郭金金, 等.蚕沙中叶酸的提取与分析检测[J].食品科技, 2009, 34 (10) :211-213.
[6]	杨玉柱, 王储炎, 焦必宁.叶酸的研究进展[J].农产品加工, 2006, 31 (5) :31-39.
[7]	梁业红.过量表达细菌的Folc和Folp基因对提高拟南芥叶酸含量的研究[D].北京:中国农业科学院, 2005:6-10.
[8]	王萍.叶酸功能性和叶酸强化食品[J].粮食与油脂, 1999, 20 (2) :53-54.
[9]	马景宏, 张旭, 王为黎.微生物分析法测定保健食品中叶酸[J].中国微生态学杂志, 2007, 19 (6) :576-577.
[10]	迟晓星, 张涛, 赵静, 等.叶酸的提取及抗氧化研究[J].现代食品科技, 2011, 27 (10) :1234-1237.
[11]	常娜宁, 姜凌, 蒲训, 等.作物叶酸检测方法的研究进展[J].中国农业科技导报, 2010, 2 (2) :44-49.
[12]	高桂花, 盛筱, 李雪冰.高效液相色谱法测定叶酸片中叶
[13]	Vahtersto L, Lehikoinen K, Ollilainen V, et al.Application of an HPLC Assay for the Determination of Folate Derivatives in some Vegetables, Fruits and Berries Consumed in Finland[J].Food Chemistry, 1997, 59 (4) :589-597.
[14]	Hefni M, 魻hrvik V, Tabekha M, et al.Folate Content in Foods Commonly Consumed in Egypt[J].Food Chemistry, 2010, 12 (1) :540-545.
[15]	Pawlosky R J, Flanagan V P.A quantitative stable-isotope LC-MS method for the determination of folic acid in fortified foods[J].Journal of Agricultural and Food Chemistry, 2001, 49 (3) :1282-1286.
[16]	左志学.高效液相色谱法测定叶酸复合维生素片叶酸含量[J].医学导报, 2010, 29 (2) :258-260.
[17]	苏耀东, 于娟, 秦立俊.间接荧光法测定奶粉中叶酸[J].理化检验, 2007, 43 (2) :105-107.
[18]	李慧芝, 许崇娟, 郭建方.微乳液增敏催化荧光光度法测定叶酸的研究[J].分析科学学报, 2009, 25 (2) :208-210.
[19]	薛月圆, 李鹏, 林勤保.小米的化学成分及物理性质的研究进展[J].中国粮油学报, 2008, 23 (3) :199-203.
[20]	刘丽萍.小米营养及小米食品的开发[J].粮油加工与食品机械, 2003 (1) :48-49.
[21]	王丽霞, 孙海峰, 赵海云, 等.山西小米资源开发利用的研究—小米营养蛋白粉制备技术[J].食品工业科技, 2007, 28 (1) :173-175.
[22]	郭丽琼, 曹秋旭, 吴厚玖.果蔬中叶酸分析方法的研究进展[J].http://www.cnki.net/kcms/detail/ll.1759.ts.20111018.1014.010.html.
[23]	秦立俊.间接荧光法和间接原子吸收光谱法测定叶酸[D].上海:同济大学, 2006:14-17.
[24]	熊绿芸, 何照范, 黄美霞.荧光法测定刺梨及其他植物材料中叶酸含量的研究[J].贵州农学院学报, 1986, 2 (2) :92-97.
[25]	郭秀珠, 黄品湖, 雷海清, 等.果蔬中叶酸的提取分离及测定方法研究[J].食品科学, 2006, 27 (3) :183-185.

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