Optimizing extraction conditions of anti-Vibrio alginolyticu substances from Anji white tea by response surface methodology

Ultrasonic wave was applied for extraction of effective component from Anji white tea.Effects of ethanol concentration, extraction time and ultrasonic power on the anti-Vibrio alginolyticu effect of the extract were investigated.On the basis of single factor investigation, response surface methodology with three factors at three levels was adopted to optimize the extraction technology of anti-Vibrio alginolyticu substances from Anji white tea.The best extraction parameters were determined by the regression analysis.Results showed that the optimum conditions of extraction were as follows:ethanol concentration 60%, extraction time 55 minutes, ultrasonic power 450W.Under the optimized conditions, total number of bacterial colony was 55CFU.