Study on the immobilization of superoxide dismutase with magnetic Fe3O4/CS microparticles
More Information
-
-
- Received Date:
February 18, 2013
-
-
Abstract
In this thesis, using Fe3O4 as magnetic source and chitosan as polymer, magnetic chitosan composite microparticles (Fe3O4 /CS) were prepared by coagulating precipitation.The magnetic Fe3O4 /CS microparticles were charectered by IR, VSM, and the size distribution of magnetic Fe3O4 /CS was tested.Using the magnetic Fe3O4/CS microparticles as carrier, superoxide dismutase (SOD) was immobilized by chemical cross-Linking.Effect of the amount of SOD, time, pH and the amount of glutaraldehyde on the activity of immobilized SOD were studied.The optimum immobilization conditions of SOD were also studied by orthogonal experiment.The optimum immobilization conditions were as follows:the amount of SOD was 100mg per 200mg magnetic chitosan microspheres, the time was 240min, the pH was 6.5 and the amount of glutaraldehyde was 0.20mL.After using four times, the activity and recovery rate of immobilized SOD were 376U/g and 56.5% respectively, which showed that the immobilized SOD had good reusability.
-
-
References
| [1] |
FRENCH S, WILSON K S.On treatment of negative intensity observations[J].Acta Crystallogr Sect A, 1978, 34:517-525.
|
| [2] |
EDEAS M, EMERIT L, KHALFOUN Y, et al.Clastogenic fators in plasma of HIV-1infected patients activate HIV-1replieation in vitro:inhibition by superoxide Dismutase[J].Free Radic Biol Med, 1997, 23 (4) :571-578.
|
| [3] |
王凤山, 张天民, 姬胜利.超氧化物歧化酶的临床应用及开发[J].中国医药工业杂志, 2011, 5 (17) :26-27.
|
| [4] |
THUST M, SCHONING M J, SCHROTH P, et al.Enzyme immobilization on planar andporous silicon substrates for biosensor applications[J].J Mol Catal B:Enzym, 1999, 7 (14) :77-83.
|
| [5] |
高大响, 贾君.大蒜SOD提取及在SOD型酸奶中的应用研究[J].食品科技, 2010, 35 (1) :220-222.
|
| [6] |
郭雪松, 孟宪军.树莓超氧化物歧化酶的提纯方法比较[J].食品工业科技, 2008, 29 (5) :210-214.
|
| [7] |
邓莉, 刘章武, 杜金平.响应面法优化鹅血中SOD热变性提纯工艺条件[J].食品工业科技, 2011, 32 (7) :270-273.
|
| [8] |
王保全, 庞晓斌, 李昭华, 等.猪血超氧化物歧化酶分离纯化工艺改进及其抗氧化活性研究[J].生物技术通报, 2010 (2) :168-172.
|
| [9] |
文连奎, 贺阳.越橘叶超氧化物歧化酶超声波提取工艺的响应面优化[J].食品科学, 2012, 33 (3) :153-158.
|
| [10] |
韩薇妍, 龚平, 田平芳.超氧化物歧化酶修饰产物结构和性能研究[J].北京化工大学学报:自然科学版, 2010, 37 (2) :117-120.
|
| [11] |
Dong-Geun L, KANAGASABAI M, Ponvel, et al.Immobilization of lipase on hydrophobic nano-sized magnetite particles[J].J Mol Catal B:Enzym, Enzymatic, 2009, 57:62-66.
|
| [12] |
李磊, 马长清, 刘飞, 等.超氧化物歧化酶的固定化及其酶学性质[J].中国生物制品学志, 2009, 22 (2) :153-155.
|
-
DownLoad: