Anti-low density lipoprotein oxidation of the crude extract and different fractions of Terminalia chebuLa Retz

The property of anti-low density lipoprotein oxidation of Terminalia chebuLa Retz(TCR) was studied in vitro Cu 2 + induction on LDL. The effective fraction of TCR on the anti -LDL oxidation was determined by thiobarbituric acid reactive substance(TBARS) guided method. All these results were showed as follows:The inhibition rates of the TCR crude extracts on LDL oxidation were 64.31%,77.22% and 82.66%,respectively. The ethyl acetate fraction possessed the most strong inhibition activity on LDL than other three fractions(p<0.05), and was the effective fraction with the same concentrations. The inhibition rates of the effective fraction(0.5,0.9 and 2.7mg/mL) were 73.19% ,81.85% and 86.29% ,respectively. The incubation periods of oxidation were delayed and degrees of oxidation were reduced by the effective fraction with different concentrations. The property of anti-low density lipoprotein oxidation of TCR was related to the total flavonoids and polyphenols in it. This study showed that TCR possessed strong anti-LDL oxidation activity and lay the foundation for further exploration on anti-atherosclerotic functional foods.