Study on purification and properties of peroxidase from shatian pomelo

In order to get a plant which was rich in peroxidase (POD ) , 15 species of plant were preliminary investigated by recirculating-catalysis flow analysis, and the Shatian pomelo peels peroxidase (SPP) whose enzymatic activity was the highest was purified by using ammonium sulfate grade precipitation and Sephadex G -200 gel chromatography. The result showed that:the POD activity was up to 15.08U in each gram of Shatianyou peels, the specific activity of purified SPP was up to 313.29U/mg, purification factor of 26.22 and yield of 43.20%. The optimum temperature and pH of SPP was 55℃ and 5.5 respectively. Its apparent Km for hydrogen peroxide and guaiacol was 0.099mmol/L and 0.864mmol/L under the optimal condition respectively.