Determination of the mean Degree of Polymerization (mDP) from raspberry seeds by Thin Layer Chromatography (TLC) and Gel Chromatography (GPC)

At room temperature, select silica gel GF254 as the stationary phase, V (toluene) :V (acetone) :V (acetic acid) =3:4:1 as the developing system, 15μL as the amount of sample application, separate raspberry seed proanthocyanidins by TLC and get three spectrums. HPLC was employed to analyze the compose and GPC was used to get the mDP from each spectrum of TLC. The composition of the spectrumⅠwas monomer material. The mDPs of spectrumⅡand Ⅲ were 2. 65 and 5. 11.