Optimization of the preparation of casein phosphopeptides by response surface methodology

Plackett -burman and response surface analysis methods were applied to optimize conditions of alkaline protease hydrolyzing casein hydrolysis based on hydrolysis the CPP (Casein Phosphopeptides) content. The results indicated that CPP yield was mainly influenced by substrate concentration, temperature and pH. Optimum hydrolysis conditions as follows:temperature 55℃ , substrate concentration 16% , pH10.8, enzyme- substrate ratio 1.5%, time 4.5h. On this condition, the hydrolysis the CPP yield was 33.2mg/mL.