Determination of hydroxymethylfurfural in pomegranate juice concentrates by solid phase extraction-ultra performance liquid chromatography

An ultra performance liquid chromatography (UPLC) method had been developed for the determination of hydroxymethylfurfural (HMF) in pomegranate juice concentrate.Samples were diluted with water, and then cleaned up with a Bond Elut ENV solid phase extraction cartridges.UPLC was performed on a UPLC HSS T3 (100mm×2.1mm, 1.8μm) using acetonitrile-water (8+92, V/V) as mobile phase.The detection wavelength was 285nm.Quantitation was carried out using an external standard method.The detection limit of 5-HMF was 0.02mg/L.The results showed good linear relationship at the range of 0.1～10.0mg/L.Recoveries spiked at 10、 20、50mg/kg ranged from 80.8%to 110.8%with relative standard deviations (RSD) less than 8.1%.The method was simple, fast and accurate and could be applied to the analysis of 5-HMF content in pomegranate juice concentrate.