Enzymatic hydrolysis of eggshell membrane and antioxidant activity of its hydrolysate in vitro

Neutral protease, alkali protease, trypsinase and papain were added to hydrolyze eggshell membrane and compared the effect.The optimal hydrolysis conditions of eggshell membrane were obtained by analyzing T-AOC (total antioxidative capacity) using orthogonal array design.The results showed that neutral protease hydrolysate showed the strongest effect, which optimal hydrolysis conditions were:substrate concentration 5%, pH 6.0, E/S 12000U/g, time 3h, tempreture 55℃.With the optimal conditions, T-AOC, scavenging DPPH radical capacity and scavenging hydroxyl radical capacity reached 1.598±0.131, 91.13%±2.80% and 83.67%±6.21%, respectively.Neutral protease hydrolysate of eggshell membrane and 0.1mol/L VCshowed a similarity in their antioxidant activity in vitro.