Research of the determination of 5 kinds of environmental estrogens residues in raw milk by solid phase extraction-high performance liquid chromatography tandem mass spectrometry

A solid phase extraction-high performance liquid chromatography tandem mass spectrometry method for simultaneous determination of environmental estrogens including estrone, estradiol, estriol, diethylstilbestrol and bisphenol A in raw milk was established.5 kinds of environmental estrogens were extracted from the raw milk sample by a NH2-SPE column, and concentrated with a rotary evaporator.The extract was analyzed by high performance liquid chromatography tandem mass spectrometric.Results showed that a good linear range from 0.01⁰.5μg/mL with correlation coefficient of above 0.9978 was obtained.The extraction recovery were 68.5%¹05.6% and the relative standard deviation were 2.56%⁷.59%.The limit of detection was 0.22⁰.56μg/L (S/N=3) .The described method was simple, sensitive and accurate.