Study on microwave-assisted extraction and DPPH free radicals scavenging capacity of polyphenols from Hypsizygus marmoreus

The extraction conditions of polyphenols from Hypsizygus marmoreus by microwave - assisted extraction (MAE) were studied with single factor design and response surface design. The effect of microwave power , microwave time and solvent amount on extraction yield (EY) were significant. ANOVA for response surface quadratic model was made. The results showed that the linear, quadratic term of the three factors and interactive term between microwave power and time were all significant. Optimized extraction conditions were obtained by regression model solution. Optimal parameters were 70% ethanol, solvent amount 15mL/g, microwave power 350W, extraction time 120s, 50℃ hot water, 2h and actual EY under the optimal conditions was 2.002mg/g. Scavenging capacity for DPPH free radicals of phenolic extract from Hypsizygus marmoreus was higher than BHA and V C . Within the test concentration range, a significant dose-effect relationship was found.