Study on polymer extraction and spectrophotometer determine of tea-polyphenols

Make use of the trait of aqueous polymer could separated two phases with solution in the presence of inorganic salt, tae-polyphenol was transferred polymer phase. In an Tris buffer medium of pH8, the content of tae-polyphenol was determined with Spectrophotometer. Good linear relationships was obtained in the range of 0.004⁰.4mmol·L-1 of tae-polyphenol per milliliter of solution (R2 =0.9999) , and recoveries were found in the range of 92.4%¹03.5%. The method was quick, accurate and handled easily.